EchoLUTION Cell Culture RNA Kit (2 x 384)
For single-step purification of total RNA from up to 1 x10⁶ human and animal cell lines (fresh, frozen, and stabilized adherent and suspension cells).
The EchoLUTION Cell Culture RNA Kit is optimized to eliminate most gDNA. For certain sensitive RNA downstream applications the gDNA Removal Mix can be used to remove residual DNA (needs to be ordered separately).
Features:
Convenience and speed: Workflow for 4 manual samples in 12 minutes and a complete 96-well plate in only 20 minutes.High sensitivity: Highly pure RNA free of contaminants and inhibitors.
Reliable results: The ultra-fast non-enzymatic lysis, that inactivates nucleases and stabilizes the nucleic acids. Resulting in RNA perfectly suited for downstream applications such as PCR and NGS.
Sustainability: Up to 60% less plastic consumption compared to other extraction methods and no usage of hazardous reagents.
Videos
Kit content:
- Lysis Buffer Cell RNA concentrate (2 x 275 mg)
- Lysis Solution Cell RNA (2 x 20 mL)
- Clearing Solution Cell RNA (7.5 mL)
- Low-TE Buffer
- Purification Plate 384 Type 4 (2)
- Elution Plate 384 (2)
- Adhesive Foil (2)
The conditioning plate is not included in the kit, as it can be used multiple times. You can purchase it here.
Also, no lysis plate is included in the kit. You can purchase a suitable one here or use any 96-well plate with a capacity of at least 300 μL per well.
Principle of the EchoLUTION Cell Culture workflow:
(demonstrated in plate format)