EchoLUTION Cell Culture DNA Kit (10)

For single-step purification of genomic and mitochondrial DNA from up to 2 x10⁶ eukaryotic cell lines and primary cells. 


Features:

Convenience and speed: Workflow complete in just 30 minutes due to reduced steps.
High sensitivity: Highly pure DNA free of contaminants and inhibitors.
Reliable results: Lysis under physiological conditions results in long and intact DNA perfectly suited for downstream applications such as PCR and NGS.
Sustainability: Up to 70% less plastic consumption compared to other extraction methods and no usage of hazardous reagents.

Kit content:

  • TurboLyse Protease Cell Culture (1 x 300 µL)
  • RNase Cell Culture (1 x 10 µL)
  • Lysis Buffer Cell Culture (1 x 650 µL)
  • Clearing Solution Cell Culture (1 x 100 µL)
  • Low-TE Buffer (1 x 1.2 mL)
  • Spin Columns Cell Culture (10 columns)

Principle of the EchoLUTION Cell Culture workflow:

(demonstrated in plate format)   

Available downloads:

Safety data sheet (SDS) and certificate of analysis (CoA) can be requested via QA@bioecho.de
„We used BioEcho’s EchoLUTION kits Tissue DNA Micro and CellCulture DNA for extraction of genomic DNA from cultured cells and from fresh and stabilized human tissue. Both kits impressed with their simplicity, speed and with stable and reproducible yields. Purified DNA could immediately be used for downstream applications such as PCR. With the CellCulture DNA Kit, we were also able to isolate plasmid DNA from eukaryotic cells for efficient subsequent transformation of E. coli.”

Wieland Fahr,
PhD student, Department of Biotechnology, TU Braunschweig, Germany


„I used the EchoLUTION Cell Culture DNA Kit for extraction of genomic DNA from mammalian cells in culture and it was quick and I obtained higher yields than with the Qiagen kit we use. I have performed a load of PCRs on the gDNA and all working fine. Great kit and will use again!”

John Robert Davis, PhD,
PostDoc, The Francis Crick Institute, London, UK
EchoLUTION Blood DNA Micro Kit
For single-step purification from dried blood spots (FTA cards). Features: Convenience and speed: Single-step purification allows gDNA extraction of DBS in 12 minutes after sample lysis.High compatibility: Suitable for extraction of genomic DNA from dried blood spots (DBS).High sensitivity: Highly pure genomic DNA free of contaminants and inhibitorsReliable results: Lysis under aqueous conditions results in long and intact DNA fragments perfectly suited for downstream applications such as PCR and NGS.Sustainability: Up to 70 % less plastic consumption compared to other extraction methods and no usage of hazardous reagents.

From US$38.70*
EchoLUTION Buccal Swab DNA Kit
For single-step purification of human or animal genomic DNA from dry buccal swab samples.  Features: Convenience and speed: Ultra-fast lysis combined with the single-step purifications allows extraction of 96 samples within 15 minutes after swab handling.High compatibility: Suitable for a wide range of dry swabs such as the Sarstedt® Forensic Swabs, Whatman® OmniSwabs, and Copan dry eSwabs®.High sensitivity: Highly pure genomic DNA free of contaminants and inhibitors.Reliable results: DNA fragments perfectly suited for downstream applications such as PCR, sequencing, and STR (short tandem repeat) analysis.Sustainability: Up to 70 % less plastic consumption due to reduction of extraction steps and use of alternate packaging material.

From US$188.10*
EchoLUTION Cell Culture RNA Kit
For single-step purification of total RNA from up to 2 x10⁶ human and animal cell lines (fresh, frozen, and stabilized adherent and suspension cells).The EchoLUTION Cell Culture RNA Kit is optimized to eliminate most gDNA. For certain sensitive RNA downstream applications the gDNA Removal Mix can be used to remove residual DNA (needs to be ordered separately). Features: Convenience and speed: Workflow for 4 manual samples in 12 minutes and a complete 96-well plate in only 20 minutes.High sensitivity: Highly pure RNA free of contaminants and inhibitors.Reliable results: The ultra-fast non-enzymatic lysis, that inactivates nucleases and stabilizes the nucleic acids. Resulting in RNA perfectly suited for downstream applications such as PCR and NGS.Sustainability: Up to 60% less plastic consumption compared to other extraction methods and no usage of hazardous reagents.

From US$364.80*
EchoLUTION FFPE DNA Kit
For single-step spin column purification of genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Features: Convenience: Simplified workflow with fewer hands-on steps than conventional kits.Speed: Optimized tissue processing combined with single-step purification leads to a protocol up to 70 % faster than established kits.High quality: Competitive DNA integrity number (DIN) and fragment size.Reliable results: High yield DNA perfectly suited for downstream applications such as PCR, qPCR, and NGS.Sustainability: Reduced plastic consumption, less hazardous liquid waste, and no use of hazardous xylene or other organic solvents like ethanol.

From US$75.20*
EchoLUTION FFPE RNA Kit
For single-step spin column purification of total RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Features: Convenience: Simplified workflow with fewer hands-on steps than conventional kits.Speed: Optimized tissue lysis combined with single-step purification leads to a 30 % faster protocol than established kits.High sensitivity: Highly pure RNA free of contaminants and inhibitors.Reliable results: High purity and competitive RNA integrity perfectly suited for downstream applications such as RT-qPCR, and RNA-seq. Sustainability: Up to 35 % less plastic consumption compared to conventional methods; no use of hazardous xylene or other organic solvents.

From US$104.80*
EchoLUTION Tissue DNA Micro Kit
For single-step purification of genomic and mitochondrial DNA from 0.1 to 10 mg of human or animal tissue (fresh, frozen, or stabilized). Features: Convenience and speed: Workflow complete in just 50 minutes due to reduced steps and short lysis timeHigh compatibility: Suitable for extraction of genomic and mitochondrial DNA from fresh, frozen, and stabilized tissue as well as challenging samples such as adipose tissue or muscleHigh sensitivity: Highly pure DNA free of contaminants and inhibitorsReliable results: Lysis under physiological conditions results in long and intact DNA perfectly suited for downstream applications such as PCR and NGS.Sustainability: Up to 70% less plastic consumption compared to other extraction methods and no usage of hazardous reagents.

From US$50.10*
EchoLUTION Viral RNA/DNA Kit (IVD)
For in-vitro diagnostic use. This product is characterized by the EchoLUTION single-step purification technology, an instant lysis without incubation for swab samples, and a fast lysis step of 10 minutes for stool samples. Together, the overall extraction time is reduced to a minimum and the workflow results in consistent performance. The product complies with the European In-Vitro Diagnostic Regulation (IVDR) and is CE-marked. The product complies with FDA regulations and is an FDA-listed in vitro diagnostic device. The product is not available in countries outside of the European Union and the United States. If you are interested in this product for countries outside the EU and the US, please contact us.Features: Convenience and speed: Single-step purification allows complete extraction of 2 x 96 swab samples within 20 minutes. Appropriate for any lab—from medium to high throughput.High compatibility: Compatible with nasopharyngeal swabs and oropharyngeal swabs and different types of non-chaotropic and chaotropic resuspension and transport media as well as for stool samples.Validation: IVDR-compliant, CE-marked, and FDA-listed. It can be integrated into diagnostic laboratory routines and ensures high-quality standards.Reliable results: With no organic solvents added and by reliable removal of other inhibitors, kit usage results in high-quality viral RNA or DNA for downstream applications like (RT-)PCR.Sustainability: Up to 70% less plastic consumption compared to other extraction methods

From US$460.50*
EchoLUTION Viral RNA/DNA Kit (RUO)
For single-step purification of viral RNA and DNA from nasopharyngeal and genital swabs (both dry and in medium), stool samples, and all types of media (non-chaotropicand chaotropic medium, PBS, and Tris). Features: Convenience and speed: Single-step purification and no incubation allows complete extraction within a few minutes.High compatibility: Compatible with nasopharyngeal swabs, genital swabs, and stool samples (dry and in medium) and all types of media like non-chaotropic and chaotropic medium, PBS, and Tris.High sensitivity: Highly pure DNA and RNA free of contaminants and inhibitorsReliable results: Inhibitor-free viral RNA/DNA results in superior downstream performance.Sustainability: Up to 70% less plastic consumption compared to other extraction methods.

From US$263.30*
RNase, DNase-free (200 kunitz unit/ml), 1 ml
For RNA removal during DNA purification.

US$114.00*
TurboLyse C (CellCulture) Protease Mix
For efficient lysis of cell culture samples.

From US$57.00*
UltraFast Lysis Cell RNA Mix
For ultra-fast lysis of cell culture samples.This product is for use with the EchoLUTION Cell Culture RNA Kit.

From US$65.00*
Spin Column Adapter for Plate Centrifuges (2 Adapters)
To centrifuge spin columns in plate centrifuges.

US$169.80*
BioEcho Cap Puncher
For efficient handling of EchoLUTION Spin Columns.

US$9.10*
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