• Enteropathogenic Viruses

    Speed up Your PCR Tests for Enteropathogenic Diagnostics

Shorten time to diagnostic results with our single-step purification

Reliable and fast diagnosis of infectious diseases is essential for providing patients with optimal care. Nucleic acid extraction is the first step in performing analyses based on RT-qPCR for determining the presence of a specific pathogen as well as monitoring the infection and the success of treatment. However, when a quick diagnosis is required, as in the case for enteropathogenic infections, time-consuming and difficult nucleic acid extraction techniques can slow down the time to result.

BioEcho helps you to meet your enteropathogenic testing challenges by providing today’s fastest and simplest viral nucleic acid extraction from stool samples. Our solutions enable diagnostic labs to easily scale up for high demands, to perform more tests in a shorter time and to get answers faster.

Get results faster

Reduce hands-on time with one-minute extraction technology.

Simplify workflows significantly

Nucleic acid extraction with half the steps of conventional methods.

Adapt throughput to your needs

Middle- and high-throughput solutions to scale up easily and provide flexibility in a cost-effective way.

Inhibitor-free nucleic acids

Highly pure viral RNA or DNA from enteropathogens for reliable and reproducible RT-qPCR tests.

Reduced consumable costs

Less consumption of consumables due to fewer workflow steps compared to other methods.

Triple the speed & halve the steps

Reliably testing samples in the shortest possible time while maintaining high sensitivity is essential for optimal patient care. Our EchoLUTION technology provides single-step nucleic acid isolation. Nucleic acids pass through the purification matrix without interaction, whereas impurities are held back and, thus, are completely removed. Different from the conventional silica-based bind–wash–elute nucleic acid extraction method, our technology allows the fastest and simplest nucleic acid extraction of enteric viruses from stool samples with the highest purity. 

With the EchoLUTION technology, only 10 minutes incubation is needed for enteropathogenic viral lysis. After a centrifugation step to prepare the column or plate, the purification is accomplished in a single one-minute centrifugation. Compared to methods based on the bind–wash–elute principle including several centrifugation steps, the EchoLUTION technology is up to three times faster.

Robust qPCR efficiency

To evaluate our EchoLUTION technology, positive stool samples from patients were stored frozen and used to determine qPCR efficiency. The samples were validated for clinical diagnosis of adenovirus and rotavirus. Results confirmed that the EchoLUTION technology exhibited suitable efficiency, confirming that no inhibitors are present in the sample after nucleic acid extraction.

(RT)-qPCR of a dilution series from nucleic acids extracted using the EchoLUTION Viral RNA/DNA Kit. (RT)-qPCR quantification cycle (Ct) values are shown on a logarithmic scale. N = 8 replicates for each dilution and virus type.

The qPCR efficiency (%) has been calculated based on the slope of the log linear regression. Samples isolated with the EchoLUTION kit exhibit a reliable qPCR efficiency. Optimal efficiency lies between 90 – 110 %.

“The Viral RNA/DNA Swab Kit from BioEcho ensures a reliable diagnosis of viral gastrointestinal infections. The EchoLUTION workflow significantly shortens the time-to-result compared to our automated magnetic-bead-based extraction and thereby contributes to fast treatment of patients.”

Dr. rer. medic. Christoph Schönfels,
Head of PCR Department, Medizinische Labor Ostsachsen MVZ GbR, Görlitz, Germany

Enteropathogenic diagnostic solutions

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Further reading

Entheropathogenic Viruses

For reliable and fast diagnosis.

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Respiratory viruses’ detection

For reliable and fast diagnostic.

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Diagnosis of enteropathogenic viruses from clinical stool samples

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